BioScience Labs provides services for evaluating product efficacy in the prevention and/or removal of biofilms using in-vitro techniques. This method represents a low fluid shear environment with an air-liquid interface, resulting in thicker biofilms. It is used to model wound systems or other medical biofilms on implants or devices.
Significance and Use
Biofilm bacteria are phenotypically different than suspended bacteria of the same genotype. Using biofilm growth reactors, biofilms are grown with specific characteristics. These characteristics can then be changed by altering the operating conditions or the engineered system.
The purpose of this test method is to grow a biofilm of Pseudomonas aeruginosa under low fluid shear and close to the air/liquid interface using the drip flow reactor (DFR) over 54 hours generating a biofilm that can be sampled on and analyzed. The biofilms grown can be treated insitu or harvested and treated individually.
This test method gives the parameters required to repeatedly grow Pseudomonas aeruginosa biofilms with a continuous flow of nutrients under low fluid shear conditions close to the air/liquid interface in a reactor.
This test method uses a drip flow reactor (DFR) which is a plug flow reactor with a laminar flow resulting in low fluid shear. Since the reactor is versatile biofilms of different species can be grown as well, although this requires changing the operational parameters depending on the growth parameters of the new organism.
This test method is used in the in-vitro laboratory to demonstrate kill efficacy on biofilms, as well as the prevention of biofilm growth.