Single Tube Method
BioScience Labs provides services for evaluating product efficacy in the prevention and/or removal of biofilms using in-vitro techniques. This method is typically used in conjunction with biofilm grown in the CDC Biofilm Reactor. The method produces quantifiable results for disinfectant efficacy evaluation of treated biofilms compared to untreated controls in a closed system.
Significance and Use
Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. Using biofilm growth reactors, biofilms are grown with specific characteristics. These characteristics and the physiological environment can then be changed by altering the operating conditions or the engineered system.
This test method was designed to show bacterial reduction after exposure to disinfectant in a closed system using CDC biofilm reactors. This test method uses 50 mL conical tubes that allow for disinfectant exposure to all sides of the testing surface. For disinfectants with a larger volume of neutralizer or foaming disinfectants, 250 mL conical tubes are used. Each test includes five treated test surfaces and three untreated controls.
This test method specifies the operational parameters required to perform a quantitative liquid disinfectant efficacy test against bacterial biofilm. It was optimized and validated for Pseudomonas aeruginosa or Staphylococcus aureus biofilms but is suitable for evaluating additional bacteria grown. This method uses a closed system to treat biofilms and describes a method to harvest and analyze biofilms. Efficacy is reported as a reduction of culturable cells.
This test method is used in the in-vitro laboratory to demonstrate quantitative liquid disinfectant efficacy on biofilms.